An In vitro Analysis of the Effects of Iron Sulfate and Iron Acetate on Streptococcus mutans
Statement of problem: Dental caries is a common infectious disease induced by Streptococcus mutans (S. mutans).
Objectives: Due to the high incidence rate of dental caries and iron deficiency in the Iranian population, we have conducted this study to analyze the effects of iron acetate and iron sulfate on controlling the growth of S. mutans.
Materials and Methods: In this in vitro study, we evaluated the antibacterial effects of iron sulfate and iron acetate on S. mutans by the disk diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The results were compared to those for 0.2% chlorhexidine and penicillin as the controls.
Results: Iron sulfate had higher MIC and MBC values compared to penicillin and chlorhexidine (P<0.001). Iron acetate MIC and MBC values did not significantly differ with penicillin and chlorhexidine. The iron sulfate inhibition zones at the 25 and 50 µg/mL doses were more than those of iron acetate.
Conclusions: Iron sulfate and iron acetate solutions can inhibit the growth of S. mutans. Hence, different compounds that contain iron salts such as toothpastes, mouth washes, and food supplements can be produced to prevent dental caries and iron deficiency.
Pitts NB, Zero DT, Marsh PD, et al. Dental caries. Nature reviews Disease primers. 2017;3:17030.
Ghapanchi J, Lavaee F, Moattari A, et al. The antibacterial effect of four mouthwashes against Streptococcus mutans and Escherichia coli. J Pak Med Assoc. 2015;65:350-3.
Ghapanchi J, Bazargani A, Zariean A, et al. Evaluation of the Anti-Streptococcus mutans Potential of Petroselinum crispum, an in vitro Study.2016;15:1-8.
Rezazadeh F, Bazargani A, Roozbeh-Shahroodi J, et al. Comparison of oral Lactobacillus and Streptococcus mutans between diabetic dialysis patients with non-diabetic dialysis patients and healthy people. J Renal Inj Prev. 2016;5:148-152.
Lavaee F, Faez K, Faez K, et al. Antimicrobial and antibiofilm activity of silver, titanium dioxide and iron nano particles. Am J Dent. 2016;29:315-20.
Berlutti F, Ajello M, Bosso P, et al. Both lactoferrin and iron influence aggregation and biofilm formation in Streptococcus mutans. Biometals . 2004;17:271-8.
Shumi W, Lim J, Nam SW, et al. Fluorescence imaging of the spatial distribution of ferric ions over biofilms formed by Streptococcus mutans under microfluidic conditions. Biochip J. 2009;3119-24.
Dunning JC, Ma Y, Marquis RE.Anaerobic killing of oral streptococci by reduced, transition metal cations.Appl Environ microbiol. 1998;64:27-33.
Devulapalle KS, Mooser G. Glucosyltransferase inactivation reduces dental caries. J Dent Res. 2001;80:466-9.
Moshaveri nia M., Golfeshan F., Sabzghabaie M.Evaluation of the relationship between the secretion of ABO blood groups antigens in saliva and DMFS caries index. Sadra medical sciences journal. 2013; 1; 253-64.
Motamedifar M, Khosropanah H, Dabiri S. Antimicrobial Activity of Peganum Harmala L. on Streptococcus mutans Compared to 0.2% Chlorhexidine. J Dent (Shiraz). 2016;17:213-218.
Albokordi M, MahmodianA, EstehghaghiR, et al. Study of the Effect of Simultaneous Iron, Zinc and Folic Acid Supplementation With or Without Vitamin A and C on Hemoglobin Levels of Teenaged Girl Students in Shahinshahr City. Journal of Medical Science University of ShahisSadoghi. 2007;16(1):14-23.
Al-Shalan TA. In vitro cariostatic effects of various iron supplements on the initiation of dental caries. Saudi Dent J. 2009;21:117-22.
Alves KM, Franco KS, Sassaki KT, et al. Effect of iron on enamel demineralization and remineralization in vitro. Arch Oral Biol. 2011;56:1192-1198.
Eskndari T, Motamedi far M, Hekmat far S, et al. The effect of three iron drop on the hardness of primary teeth in cariogenic circumstance. Journal of Dental Faculty of ShahidBeheshti Medica Science University. 2011;30:275-82.
Sales-Peres SH, Pessan JP, Buzalaf MA. Effect of an iron mouthrinse on enamel and dentine erosion subjected or not to abrasion: an in situ/ex vivo study. Arch Oral Biol. 2007;52:128-32.
Pecharki GD, Cury JA, PaesLeme AF, et al. Effect of Sucrose Containing Iron(II) on Dental Biofilm and Enamel Demineralization in situ. Caries Res .2005; 39:123-9.
Ribeiro CC, Ccahuana-Vásquez RA, Carmo CD. et al. The effect of iron on Streptococcus mutans biofilm and on enamel demineralization. Braz Oral Res. 2012;26:300-5 .
Kaur P, Singh H, Khatri A, Aulakh KS. Evaluation and comparison of short term side effects of 0.2% and 0.12%. chlorhexidine mouthwash. JAMDSR. 2015;3:26-28.
Poorshahidi S, Davarmanesh M, Rezazadeh F, et al. In vitro inhibitory effects of Chlorhexidine and Persica mouthwashes on HSV-1 compared with acyclovir. J Isfahan Dent Sch. 2011;7:59-67.
Pourshahidi S, Rezazadeh F, Motamedifar M, et al. In Vitro Comparative Study on Antiherpetic Effect of Chlorhexidine and Persica Mouthwashes with Acyclovir. J Basic Applied Scien. 2012;8:286-90.
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